Saturday, May 9, 2015

Our mini-paper introducing iMate Protocol

The modified protocol from my unit is introduced in a mini-paper published online in the journal Biotechniques.

Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing
Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu Tanegashima, and Shigehiro Kuraku
BioTechniques 58:253-257 (May 2015) doi 10.2144/000114288

Therein, we also introduce our strategy to get 80nt-long, 127nt-long or 171nt-long reads using only SBS kits for 50 cycles with HiSeq Rapid Run mode (v1).